| id |
ijac202119201 |
| authors |
Gumuskaya, Gizem |
| year |
2021 |
| title |
Multimaterial bioprinting—minus the printer: Synthetic bacterial patterning with UV-responsive genetic circuits |
| source |
International Journal of Architectural Computing 2021, Vol. 19 - no. 2, 121–141 |
| summary |
In this paper, we argue that synthetic biology can help us employ living systems’ unique capacity for self-construction and biomaterial production toward developing novel architectural fabrication paradigms, in which both the raw material production and its refinement into a target structure can be merged into a single computational process embedded in the living structure itself. To demonstrate, here we introduce bioPheme, a novel biofabrication method for engineering bacteria to build biomaterial(s) of designer’s choice into arbitrary 2D geometries specified via transient UV tracing. To this end, we present the design, construction, and testing of the enabling synthetic DNA circuit, which, once inserted into a bacterial colony, allows the bacteria to execute spatial computation by interacting with one another based on the if-then rules encoded in this circuit. At the heart of this genetic circuit is a pair of UV sensor – actuator, and a pair of cell-to-cell signal transmitter – receptor modules, created with genes extracted from the virus ? Phage and marine bacterium Vibrio fischeri, respectively. These modules are wired together to help designers engineer bacteria to build macro-scale structures with seamlessly integrated biomaterials, thereby bridge the molecular and architectural scales. In this way, a bacterial lawn can be programmed to produce different objects with complementary biomaterial compositions, such as a biomineralized superstructure and an elastic tissue filling in-between. In summary, this paper focuses on how scientists’ increasing ability to harness the innate computational capacity of living cells can help designers create self-constructing structures for architectural biofabrication. Through the discussions in this paper, we aim to initiate a shift in today’s biodesign practices toward a greater appreciation and adoption of bottom-up governance of living structures. We are confident that such a paradigm shift will allow for more efficient and sustainable biofabrication systems in the 4th industrial revolution and beyond. |
| keywords |
Synthetic biology, architecture, optogenetics, design computation, genetic circuits, biofabrication, synthetic morphogenesis, computational fabrication, architectural fabrication, biodesign |
| series |
journal |
| email |
|
| references |
Content-type: text/plain
|
Andrianantoandro E, Basu S, Karig DK, et al. (2006)
 Synthetic biology: new engineering rules for an emerging discipline
, Mol Syst Biol. Epub ahead of print May. DOI: 10.1038/msb4100073
|
|
|
|
|
Antonelli P and Museum of Modern Art (New York, NY) (2008)
Design and the elastic mind
, New York: Museum of Modern Art: Distributed in the U.S. and Canada by D.A.P./Distributed Art Publishers.
|
|
|
|
|
Bassler BL. (1999)
How bacteria talk to each other: regulation of gene expression by quorum sensing
, Curr Opin Microbiol1999; 2: 582–587.
|
|
|
|
|
Basu S, Gerchman Y, Collins CH, et al. (2005)
A synthetic multicellular system for programmed pattern formation
, Nature; 434(7037): 1130–1134.
|
|
|
|
|
Basu S, Mehreja R, Thiberge S, et al. (2004)
Spatiotemporal control of gene expression with pulse-generating networks
, Proc Natl Acad Sci; 101: 6355–6360.
|
|
|
|
|
Camazine S. (2001)
Self-organization in biological systems
, Princeton, N.J: Princeton University Press.
|
|
|
|
|
Church GM, Elowitz MB, Smolke CD, et al. (2014)
Realizing the potential of synthetic biology
, Nat Rev Mol Cell Biol; 15(4): 289–294.
|
|
|
|
|
Dagani R. (1991)
Natural biomineralization mimicked in lab
, Chem Eng News; 69: 33–34.
|
|
|
|
|
Danino T, Mondragón-Palomino O, Tsimring L, et al. (2010)
A synchronized quorum of genetic clocks
, Nature; 463: 326–330.
|
|
|
|
|
Davies JA. (2008)
Synthetic morphology: prospects for engineered, self-constructing anatomies: synthetic morphology
, J Anat; 212(6): 707–719.
|
|
|
|
|
Davies JA. (2013)
Mechanisms of morphogenesis
, 2nd ed. Amsterdam: Elsevier/AP.
|
|
|
|
|
Endy D. (2005)
Foundations for engineering biology
, Nature; 438(7067): 449–453.
|
|
|
|
|
Felton S, Tolley M, Demaine E, et al. (2014)
A method for building self-folding machines
, Science; 345: 644–646.
|
|
|
|
|
Fenno L, Yizhar O and Deisseroth K. (2011)
The development and application of optogenetics
, Annu Rev Neurosci; 34: 389–412.
|
|
|
|
|
Fernandez-Rodriguez J, Moser F, Song M, et al. (2017)
Engineering RGB color vision into Escherichia coli
, Nat Chem Biol; 13: 706–708.
|
|
|
|
|
Ginsberg AD. (2014)
Synthetic aesthetics: investigating synthetic biology’s designs on nature
, Cambridge, Mass: MIT Press.
|
|
|
|
|
Glock P, Ramm B, Heermann T, et al. (2019)
Stationary patterns in a two-protein reaction-diffusion system
, ACS Synth Biol; 8: 148–157.
|
|
|
|
|
Grant RG. (2017)
Flight: the complete history of aviation
, 6th ed. New York: DK Publishing.
|
|
|
|
|
Gumuskaya G. (2018)
Form from within: scaling up self-constructing biological architectures through a novel application of synthetic morphogenesis
, Diss. Massachusetts Institute of Technology.
|
|
|
|
|
Hawkes E, An B, Benbernou NM, et al. (2010)
Programmable matter by folding
, Proc Natl Acad Sci; 107: 12441–12445.
|
|
|
|
| last changed |
2024/04/17 14:29 |
|
